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An alternative method to isolate protease and phospholipase A2 toxins from snake venoms based on partitioning of aqueous two-phase systems J. Venom. Anim. Toxins incl. Trop. Dis.
Gómez,GN; Nerli,BB; Acosta,OC; Picó,GA; Leiva,LCA.
Snake venoms are rich sources of active proteins that have been employed in the diagnosis and treatment of health disorders and antivenom therapy. Developing countries demand fast economical downstream processes for the purification of this biomolecule type without requiring sophisticated equipment. We developed an alternative, simple and easy to scale-up method, able to purify simultaneously protease and phospholipase A2 toxins from Bothrops alternatus venom. It comprises a multiple-step partition procedure with polyethylene-glycol/phosphate aqueous two-phase systems followed by a gel filtration chromatographic step. Two single bands in SDS-polyacrylamide gel electrophoresis and increased proteolytic and phospholipase A2 specific activities evidence the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Partition; Snake toxins; Isolation; Phospholipase A2; Proteases.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000300008
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Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system BABT
Bezerra,Raquel Pedrosa; Borba,Fernanda Katharine Sousa Lins; Moreira,Keila Aparecida; Lima-Filho,José Luis; Porto,Ana Lúcia Figueiredo; Chaves,Adilson Castro.
Studies were carried out on the partition of amylase from Bacillus subtilis in a minimal medium at 37 ºC and 110 rpm. Enzyme recovery was carried out in aqueous two-phase system PEG-Phosphate salt were carried out. The best purification factor (5.4) was obtained in system PEG 1000 (16.7% w/w) with potassium phosphate (14.8% w/w), at pH 6.0, resulting in a recovery of 45.2% activity enzymatic in the salt-rich phase.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amylases; Aqueous two-phase systems; Bacillus subtilis; Partition; Poly (ethylene glycol).
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500003
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The biogeochemistry of mercury at the sediment water interface in the Thau lagoon. 1. Partition and speciation ArchiMer
Muresan Paslaru, Bogdan; Cossa, Daniel; Jezequel, D; Prevot, F; Kerbellec, S.
Solid sediment, pore and epibenthic waters were collected from the Thau lagoon (France) in order to study the post depositional partition and mobility of mercury and monomethylmercury in an organic rich sediment. Total Hg (HgT) and monomethymercury (MMHg) profiles were produced in both dissolved and solid phases. The distribution of HgT in the solid appeared to be related to the historical changes in the Hg inputs into the lagoon. HgT was in equilibrium between solid and solution in the sulfidic part of the cores, with a mean log Kd of 4.9 +- 0.2. The solid appeared to be a source of HgT for pore water in the upper oxic to suboxic parts of the cores. The MMHg represented a small fraction of HgT: 3-15 % and 0.02-0.80 % in the dissolved and in the solid...
Tipo: Text Palavras-chave: Fluxes; Partition; Sediment; Lagoon; Methylmercury; Mercury.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2006/publication-2083.pdf
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